C7898

Sigma

 

Concanavalin A from Canavalia ensiformis (Jack bean)

ferritin conjugate, Type IV, buffered aqueous glycerol solution

Synonym:Con A
CAS Number:11028-71-0
MDL number:MFCD00071069

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Description

Analysis NoteWhere reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol ActionsCon A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
PackagingPackage size based on protein content
Physical formSolution in 50% glycerol containing 0.3 M NaCl, 0.01 M sodium phosphate, 0.06 M CaCl2, 0.06 mM MnCl2 and 0.02% sodium azide as preservative

Properties

typeType IV
formbuffered aqueous glycerol solution
concentration5-10 mg/mL protein (Biuret)
extent of labeling~1 mol ferritin per mol lectin
total impurities carbohydrate, essentially free
conjugateferritin conjugate
storage temp.−20°C

Safety

Risk Statements36/37/38
Safety Statements26
WGK Germany2

References

referenceReeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)
 Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)
 Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)
 , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
MerckMerck 13,2521